Bogor, 17-10-2018       Indonesia Version

 Technique of microencapsulating spermatozoa is used to enclose a group of spermatozoa cells in a semi-permeable membrane. It will solve the problem related to the timing of AI due to variation in estrous and ovulation time.

 Artificial Insemination (AI) is a reproduction technology most applicative for a mating system in dairy cattle farming in Indonesia. The efficiency of AI would increase with the application of reproduction technologies such as artificial insemination, embryo transfer with its supporting techniques such as semen preservation, estrous synchronization, and ovulation synchronization.

Application of hormones to manipulate reproduction is generally conducted through intramuscular injection, implant using CIDR, sponge, insert. Intranasal application using spray on animals is not commonly practiced, though it has been done in human.

Spray application is expected to reduce stress and increase efficiency because it uses a smaller dosage when the target hormone is in the brain. The development of nano technology makes it possible to apply hormone in a smaller dosage, and in some cases for hormones with a short half-life such as FSH and LH the technique can be applied in order to control their release and create a chance to improve efficiency.

A series of experiments has been conducted to develop hormone nano-particles technique in order to manipulate reproduction.

In 2015, the activity was directed to develop nanoparticles of human chorionic gonadotrophin (hCG) hormone to induce ovulation. In 2016, research focus was directed to develop nanoparticles of prostaglandin hormone to be applied through nasal spray and intramuscular for heat synchronization.

Technique of microencapsulating spermatozoa is used to enclose a group of spermatozoa cells in a semi-permeable membrane. The membrane is to protect spermatozoa so that their viability can be maintained longer in the female reproductive tract.

The success of micro encapsulation technique with a relatively lower cost (extra cost only 1000/straw), and the success of freezing process of microencapsulated semen according to SNI standard (minimal PTM of 40%) provided an opportunity for microencapsulated semen to be applied.

The success of AI is the key to the development of reproduction manipulation such as microencapsulation of spermatozoa. The availability of data on good insemination technique and timing, position, and other information becomes very important. In 2016, AI was focused on the peak of heat (16 hours earlier than conventional time) in which from the previous research encapsulated semen showed better results than control.

In 2016, AI trial using encapsulated semen was conducted in KUD Tandangsari. The total of 150 straws of microencapsulated semen and 150 straws of control semen were sent to smallholder farmers with early insemination for encapsulated semen and standard procedure for frozen semen. The results were expected to confirm results from previous study on early insemination.

The microencapsulated semen had a possibility to result in pregnancy when inseminated earlier. The next trial will be conducted on animals by observing the onset of estrous, at which AI using microencapsulated semen will show higher pregnancy rate than control. Results will be confirmed with larger recipients.